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inverted delta vision restoration microscopy system  (Applied Precision Inc)

 
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    Applied Precision Inc inverted delta vision restoration microscopy system
    NS1 and RIG-I/MDA5 interactions based on a BiFC system and colocalization assay. (A) Schematic of the basic principles of the BiFC system. N- and C-terminal fragments of Venus fluorescent protein are fused to proteins A and B, respectively. The interaction between A and B brings the N- and C-terminal fragments of Venus in proximity to reconstitute the intact fluorescent protein. (B) 293T cells were cotransfected with V-N-NS1 and V-C-RIG-I/MDA5 plasmids and incubated at 37°C for 12 h. Plasmids V-N-p53 and V-C-T-tag were used as the positive controls and cotransfected into the cells, while V-N-mp53 and V-C-mT-tag were used as the negative controls and cotransfected into the cells. Fluorescent images generated by BiFC in the cells were visualized using an inverted <t>Delta</t> Vision Restoration <t>microscopy</t> system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. HA-NS1 and FLAG-RIG-I/MDA5 plasmids were cotransfected into Vero cells. The cells were then incubated with both mouse anti-HA Ab and rabbit anti-FLAG Ab, followed by Cy3-conjugated goat anti-rabbit IgG and FITC-conjugated goat anti-mouse IgG secondary Abs. Then, cells were stained with DAPI and observed under a confocal microscope (Nikon A1 MP Storm). Colocalization of HA-NS1 (green) and FLAG-RIG-I/MDA5 (red) appears as yellow. (E) The percentage of cells with colocalization of NS1 and RIG-I/MDA5 was quantified in a number of fields.
    Inverted Delta Vision Restoration Microscopy System, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+delta+vision+restoration+microscopy+system/inverted+delta+vision+restoration+microscopy+system/pmc05571242-186-13-18
    Average 90 stars, based on 1 article reviews
    inverted delta vision restoration microscopy system - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5"

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5

    Journal: Journal of Virology

    doi: 10.1128/JVI.02396-16

    NS1 and RIG-I/MDA5 interactions based on a BiFC system and colocalization assay. (A) Schematic of the basic principles of the BiFC system. N- and C-terminal fragments of Venus fluorescent protein are fused to proteins A and B, respectively. The interaction between A and B brings the N- and C-terminal fragments of Venus in proximity to reconstitute the intact fluorescent protein. (B) 293T cells were cotransfected with V-N-NS1 and V-C-RIG-I/MDA5 plasmids and incubated at 37°C for 12 h. Plasmids V-N-p53 and V-C-T-tag were used as the positive controls and cotransfected into the cells, while V-N-mp53 and V-C-mT-tag were used as the negative controls and cotransfected into the cells. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. HA-NS1 and FLAG-RIG-I/MDA5 plasmids were cotransfected into Vero cells. The cells were then incubated with both mouse anti-HA Ab and rabbit anti-FLAG Ab, followed by Cy3-conjugated goat anti-rabbit IgG and FITC-conjugated goat anti-mouse IgG secondary Abs. Then, cells were stained with DAPI and observed under a confocal microscope (Nikon A1 MP Storm). Colocalization of HA-NS1 (green) and FLAG-RIG-I/MDA5 (red) appears as yellow. (E) The percentage of cells with colocalization of NS1 and RIG-I/MDA5 was quantified in a number of fields.
    Figure Legend Snippet: NS1 and RIG-I/MDA5 interactions based on a BiFC system and colocalization assay. (A) Schematic of the basic principles of the BiFC system. N- and C-terminal fragments of Venus fluorescent protein are fused to proteins A and B, respectively. The interaction between A and B brings the N- and C-terminal fragments of Venus in proximity to reconstitute the intact fluorescent protein. (B) 293T cells were cotransfected with V-N-NS1 and V-C-RIG-I/MDA5 plasmids and incubated at 37°C for 12 h. Plasmids V-N-p53 and V-C-T-tag were used as the positive controls and cotransfected into the cells, while V-N-mp53 and V-C-mT-tag were used as the negative controls and cotransfected into the cells. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. HA-NS1 and FLAG-RIG-I/MDA5 plasmids were cotransfected into Vero cells. The cells were then incubated with both mouse anti-HA Ab and rabbit anti-FLAG Ab, followed by Cy3-conjugated goat anti-rabbit IgG and FITC-conjugated goat anti-mouse IgG secondary Abs. Then, cells were stained with DAPI and observed under a confocal microscope (Nikon A1 MP Storm). Colocalization of HA-NS1 (green) and FLAG-RIG-I/MDA5 (red) appears as yellow. (E) The percentage of cells with colocalization of NS1 and RIG-I/MDA5 was quantified in a number of fields.

    Techniques Used: Incubation, Generated, Microscopy, Software, Staining

    Related Articles

    Generated:

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: .. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. ..

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: .. The fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. ..

    Bimolecular Fluorescence Complementation Assay:

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: .. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. ..

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: .. The fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. ..

    Microscopy:

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: .. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. ..

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: .. The fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. ..

    Article Title: In vivo imaging of protein–protein and RNA–protein interactions using novel far-red fluorescence complementation systems
    Article Snippet: .. Cells were imaged with an inverted Delta Vision Restoration Microscopy System (Applied Precision, WA, USA) equipped with a cooled CCD camera. ..

    Software:

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: .. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. ..

    other:

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
    Article Snippet: Fluorescent images generated by BiFC in the 841 cells were visualized using an inverted Delta Vision Restoration Microscopy System 842 (Applied Precision, WA, USA) with excitation and emission wave lengths of 500/24 nm 843 and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using 844 on June 28, 2017 by U N IV O F C A LIF S A N D IE G O http://jvi.asm .org/ D ow nloaded from 33 Volocity software; a.u., arbitrary units. (D) Co-localization of NS1 and RIG-I/MDA5.



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    inverted delta vision restoration microscopy system  (Applied Precision Inc)
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    Applied Precision Inc inverted delta vision restoration microscopy system
    NS1 and RIG-I/MDA5 interactions based on a BiFC system and colocalization assay. (A) Schematic of the basic principles of the BiFC system. N- and C-terminal fragments of Venus fluorescent protein are fused to proteins A and B, respectively. The interaction between A and B brings the N- and C-terminal fragments of Venus in proximity to reconstitute the intact fluorescent protein. (B) 293T cells were cotransfected with V-N-NS1 and V-C-RIG-I/MDA5 plasmids and incubated at 37°C for 12 h. Plasmids V-N-p53 and V-C-T-tag were used as the positive controls and cotransfected into the cells, while V-N-mp53 and V-C-mT-tag were used as the negative controls and cotransfected into the cells. Fluorescent images generated by BiFC in the cells were visualized using an inverted <t>Delta</t> Vision Restoration <t>microscopy</t> system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. HA-NS1 and FLAG-RIG-I/MDA5 plasmids were cotransfected into Vero cells. The cells were then incubated with both mouse anti-HA Ab and rabbit anti-FLAG Ab, followed by Cy3-conjugated goat anti-rabbit IgG and FITC-conjugated goat anti-mouse IgG secondary Abs. Then, cells were stained with DAPI and observed under a confocal microscope (Nikon A1 MP Storm). Colocalization of HA-NS1 (green) and FLAG-RIG-I/MDA5 (red) appears as yellow. (E) The percentage of cells with colocalization of NS1 and RIG-I/MDA5 was quantified in a number of fields.
    Inverted Delta Vision Restoration Microscopy System, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inverted+delta+vision+restoration+microscopy+system/inverted+delta+vision+restoration+microscopy+system/pmc05571242-186-13-18
    Average 90 stars, based on 1 article reviews
    inverted delta vision restoration microscopy system - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    NS1 and RIG-I/MDA5 interactions based on a BiFC system and colocalization assay. (A) Schematic of the basic principles of the BiFC system. N- and C-terminal fragments of Venus fluorescent protein are fused to proteins A and B, respectively. The interaction between A and B brings the N- and C-terminal fragments of Venus in proximity to reconstitute the intact fluorescent protein. (B) 293T cells were cotransfected with V-N-NS1 and V-C-RIG-I/MDA5 plasmids and incubated at 37°C for 12 h. Plasmids V-N-p53 and V-C-T-tag were used as the positive controls and cotransfected into the cells, while V-N-mp53 and V-C-mT-tag were used as the negative controls and cotransfected into the cells. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. HA-NS1 and FLAG-RIG-I/MDA5 plasmids were cotransfected into Vero cells. The cells were then incubated with both mouse anti-HA Ab and rabbit anti-FLAG Ab, followed by Cy3-conjugated goat anti-rabbit IgG and FITC-conjugated goat anti-mouse IgG secondary Abs. Then, cells were stained with DAPI and observed under a confocal microscope (Nikon A1 MP Storm). Colocalization of HA-NS1 (green) and FLAG-RIG-I/MDA5 (red) appears as yellow. (E) The percentage of cells with colocalization of NS1 and RIG-I/MDA5 was quantified in a number of fields.

    Journal: Journal of Virology

    Article Title: West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5

    doi: 10.1128/JVI.02396-16

    Figure Lengend Snippet: NS1 and RIG-I/MDA5 interactions based on a BiFC system and colocalization assay. (A) Schematic of the basic principles of the BiFC system. N- and C-terminal fragments of Venus fluorescent protein are fused to proteins A and B, respectively. The interaction between A and B brings the N- and C-terminal fragments of Venus in proximity to reconstitute the intact fluorescent protein. (B) 293T cells were cotransfected with V-N-NS1 and V-C-RIG-I/MDA5 plasmids and incubated at 37°C for 12 h. Plasmids V-N-p53 and V-C-T-tag were used as the positive controls and cotransfected into the cells, while V-N-mp53 and V-C-mT-tag were used as the negative controls and cotransfected into the cells. Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5. HA-NS1 and FLAG-RIG-I/MDA5 plasmids were cotransfected into Vero cells. The cells were then incubated with both mouse anti-HA Ab and rabbit anti-FLAG Ab, followed by Cy3-conjugated goat anti-rabbit IgG and FITC-conjugated goat anti-mouse IgG secondary Abs. Then, cells were stained with DAPI and observed under a confocal microscope (Nikon A1 MP Storm). Colocalization of HA-NS1 (green) and FLAG-RIG-I/MDA5 (red) appears as yellow. (E) The percentage of cells with colocalization of NS1 and RIG-I/MDA5 was quantified in a number of fields.

    Article Snippet: Fluorescent images generated by BiFC in the cells were visualized using an inverted Delta Vision Restoration microscopy system (Applied Precision, WA, USA) with excitation and emission wavelengths of 500/24 nm and 542/27 nm, respectively. (C) Quantitative analysis of the BiFC efficiency using Volocity software; a.u., arbitrary units. (D) Colocalization of NS1 and RIG-I/MDA5.

    Techniques: Incubation, Generated, Microscopy, Software, Staining